Chemical constituents and antioxidant activity from the rhizomes of Zingiber Cassumunar

Nurul Amila Fadhlin Baharuddin, Hasnah Mohd Sirat


Zingiber cassumunar is one of the species belongs to the genus of Zingiber in the Zingiberaceace family. Hydrodistillation of fresh rhizomes afforded 2.83% of essential oil as a pale yellow liquid with pungent smell. Chemical compositions of the oils were determined by gas chromatography and gas chromatography-mass spectrometry techniques. Twenty-nine compounds were found in the rhizome essential oil which contributed to 78.74% of the total oil. The compounds have been classified as monoterpenes (75.9%), sesquiterpenes (1.8%) and ester (0.8%). The major constituents were found to be terpinen-4-ol (31.35%) (1)and sabinene (23.37%)(2). The dried rhizome of Z. cassumunar was also extracted with chloroform using Soxhlet extraction for twenty hours. Fractionation of the crude extract using silica gel vacuum liquid chromatography, followed by repeated purification using silica gel gravity column chromatography afforded two compounds. The structure of these compounds were elucidated by infrared and nuclear magnetic resonance spectroscopies as trans-1-(3,4-dimethoxyphenyl)butadiene (3)and trans-4-(3,4-dimethoxyphenyl)but-3-en-1-yl acetate(4). DPPH antioxidant screening using DPPH free radical scavenging activity showed that the essential oil of the rhizome of Z. cassumunar gave weak antioxidant property and chloroform crude extract gave positive antioxidant result at higher concentration.


Full Text:



Ghasemzadeh, A., Jaafar, H. Z., andRahmat, A. (2010). Antioxidant activities, Total phenolics and flavonoids content in two varieties of Malaysia young ginger (Zingiberofficinale Roscoe). Molecules. 15, 4324-4333.

Mohamad, R. (2013). Quality control methods for some Zingiberaceous plants from Indonesia using liquid chromatography combined with chemometrics. Doctoral dissertation. 1-53.

Ubonnuch, C., Ruangwises, S., Gritsanapan, W., andRuangwises, N. (2013). Total and Inorganic Arsenic Contents in Some Edible Zingiberaceous Rhizomes in Thailand. Evidence-Based Complementary and Alternative Medicine. 13, 1-7.

Ubonnuch, C., Ruangwises, S., Gritsanapan, W., &Ruangwises, N. (2013). Total and Inorganic Arsenic Contents in Some Edible Zingiberaceous Rhizomes in Thailand. Evidence-Based Complementary and Alternative Medicine. 13, 1-7.

Habsah, M., Amran, M., Mackeen, M.M., Lajis, N.H., Kikuzaki, H., Nakatani, N., Rahman, A.A., Ghafar, and Ali, A.M. (2000). Screening of Zingiberaceace Extracts for Antimicrobial and Antioxidant Activities. Journal of Ethnopharmacology72, 403-410.

Parthasarathy, V.A., Zachariah, T.J., andChempakam, B. (2008). Ginger. Chemistry of Spices. 5, 70-96.

Bua-In, S., Paisooksantivatana, Y., Weimer, B. C., andChowpongpang, S. (2014). Molecular cloning and expression levels of the monoterpene synthase gene (ZMM1) in Cassumunar ginger (Zingibermontanum (Koenig) Link ex Dietr.). Archives of Biological Sciences. 4, 1321-1331.

Hamirah, M.N., Sani, H.B., Boyce, P.C., andSim, S.L. (2010). Micropropagation of red ginger (Zingibermontanum Koenig), a medicinal plant. In Proceedings Asia Pacific Conference on Plant Tissue and AgribiotechnologyJournal of MolecularBiology&Biotechnology.18, 127-130.

Sukatta, U., Rugthaworn, P., Punjee, P., Chidchenchey, S., and Keeratinijakal, V. (2009). Chemical composition and physical properties of oil from Plai (ZingibercassumunarRoxb.) obtained by hydro distillation and hexane extraction. Kasetsart Journal. (National Science.)43, 212-217.

Bua-in, S., andPaisooksantivatana, Y. (2009). Essential oil and antioxidant activity of cassumunarginger (Zingiberaceae: Zingibermontanum (Koenig) Link ex Dietr.) collected from various parts of Thailand. Kasetsart Journal: (Natural Science). 43, 467-475.

Saat, R., (1999). Kandungan Kimia danbioaktivititiga species Zingiberaceace. (Master’s Thesis, Faculty of Science,UniversitiTeknologi Malaysia,).

Masuda, Toshiya, and Akiko Jitoe. (1995). Phenylbutenoid monomers from the rhizomes of Zingibercassumunar. Phytochemistry.39, 459-461.


  • There are currently no refbacks.

Copyright (c) 2016 eProceedings Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Copyright © 2016 Department of Chemistry, Universiti Teknologi Malaysia.

Disclaimer : This website has been updated to the best of our knowledge to be accurate. However, Universiti Teknologi Malaysia shall not be liable for any loss or damage caused by the usage of any information obtained from this web site.
Best viewed: Mozilla Firefox 4.0 & Google Chrome at 1024 × 768 resolution.