Deacetylation of Chitin Isolated from Fermented Prawn Waste to produce Chitosan using Autoclave method

Zainoha Zakaria, Tze Song Bong, Marshifah Jamaludin


A partially purified chitin was isolated from fermented prawn waste.  Chitin was further deacetylated using autoclave method under condition of 121℃ and 101.kPa to form chitosan with the objective of reducing the deacetylation time and cost of production. The effect of NaOH concentrations and various steeping modes on the degree of deacetylation (DD) of chitosan were evaluated. Degree of deacetylation of chitosan was determined using FTIR spectroscopy. Chitosan film was not formed when chitin was deacetylated in 40% and 45% (w/w) NaOH solution. Effective chitosan film formation was observed only when 50% (w/w) NaOH was used. Result showed that chitin was successfully deacetylated using autoclave and reaction time of 20 minutes to form chitosan of up to 84% DD. The DD also increases with steeping time and a double steeping mode was able to increase up to 91% DD of chitosan.

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